Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli
Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli
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The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in click here nearly 20% of men’s fine perfumery.The biotechnological production of such fragrant sesquiterpenes is a promising alternative towards sustainability; nevertheless, product recovery from fermentation is one of the main constraints.In an effort to improve the (+)-zizaene recovery from a metabolically-engineered Escherichia coli, we developed an integrated bioprocess by coupling fermentation and (+)-zizaene recovery using adsorber extractants.
Initially, (+)-zizaene volatilization was confirmed from cultivations with no extractants but application of liquid−liquid phase partitioning cultivation (LLPPC) improved (+)-zizaene recovery nearly 4-fold.Furthermore, solid−liquid phase partitioning cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion HP20 reached the highest recovery.Bioprocess was scaled up to 2 L bioreactors and in situ recovery configurations integrated to fermentation were evaluated.
External recovery configuration was performed with an expanded bed adsorption column and improved (+)-zizaene titers 2.5-fold higher than LLPPC.Moreover, internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 2.
2-fold, whereas adsorption velocity was determined as critical parameter for recovery efficiency.Consequently, IRC sofia barclay sexy improved the (+)-zizaene titer 8.4-fold and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 211.
13 mg L−1 and productivity of 3.2 mg L−1 h−1.This study provides further knowledge for integration of terpene bioprocesses by in situ product recovery, which could be applied for many terpene studies towards the industrialization of fragrant molecules.